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Re: areatree results: LB cable length

Posted by Albert Cardona-2 on Jan 21, 2014; 12:35am
URL: http://imagej.273.s1.nabble.com/areatree-results-LB-cable-length-tp5006194p5006196.html

The Lower Bound (LB) cable length is a way to approximate the minimal
possible cable length that the tree could have. Consists of measuring as
straight lines the distance between end node and branch node, branch node
to branch node, and branch node to root, and adding them all up.

The main effect of the LB measurement is that the jitter from the human's
innaccuracy and the jitter from the image registration errors are almost
completely removed. This is particularly important for EM reconstructions
from serial sections; if you use light-microscopy image stacks the jitter
cannot be much and is likely not significant.

There is an example of this measurement in supplemental figure 15, and an a
schematic of its measurement in suppl. figure 16 of this paper:

http://www.nature.com/nmeth/journal/v9/n7/full/nmeth.2072.html

"Elastic volume reconstruction from series of ultra-thin microscopy
sections"
Stephan Saalfeld, Richard Fetter, Albert Cardona & Pavel Tomancak
Nature Methods 9, 717–720 (2012) doi:10.1038/nmeth.2072

In there, we demonstrate how better image registration brings the cable
length of a hand-reconstructed neuronal arbor closer to the lower bound
measurement. The latter stays nearly constant for all image registration
techniques used, which endorses its robustness.

Albert



2014/1/20 Luisa de Vivo <[hidden email]>

> Hi,
>
> does anybody know the difference between Cable length and LB Cable length
> in the Areatree results window of TrakEM2?
> Thank you very much,
>
> Luisa
>
>
> --
> Luisa de Vivo, PhD
> Department of Psychiatry
> University of Wisconsin
> 6001 Research Park Boulevard
> Madison WI 53719
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>



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http://www.ini.uzh.ch/~acardona/

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