fluorescence qunatification comparison

Posted by Judy Trogadis-2 on Jan 26, 2014; 6:02pm
URL: http://imagej.273.s1.nabble.com/fluorescence-qunatification-comparison-tp5006287.html

Hi All

I am comparing surface receptor staining of 2 sets of fluorecently stained samples. The first is a control sample, the second set has been treated and we are looking at potential clustering of the receptors following treatment.

How can I quantitate this? If clustered, simple thresholding will just show a smaller number of stained pixels, it will not indicate a shift to fewer but brighter pixels. Or can it?

Thank you,

Judy


Judy Trogadis
Bio-Imaging Coordinator
Keenan Research Centre, St. Michael's
209 Victoria Street
Toronto M5B 1T8, Canada
office: 416-864-6060 ext. 77612
imaging facility:    ext. 77434
cell:  416-254-9330
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