Re: SKM Cross sectional area measurement

Posted by stevefaulkner on Jan 30, 2014; 11:21am
URL: http://imagej.273.s1.nabble.com/SKM-Cross-sectional-area-measurement-tp5006330p5006338.html

Yes they are all saved as tif files! The aim is to generate a CSA for each cell within the image, but ignoring those that come ito contact with the edges. So far I have been doing this by creating and ROI and then manually measuring a selections of cells, which is less than ideal! As you suggest, I have been doing this using the green channel alone, I just wanted to show a complete image as I'll also be analysing the density and activation of a number of other cell within the image.

When I have tried to threshold the image, the problem that I get is that the fluoro isn't uniform throughout, so each cell often becomes grainy and is no longer recogonised as a single entity as defined by the cell membrane.

I will have a number of images to analyse but so far they are just stored as each composite image (each filter) of the total overlay in a single file from a single field of view, all within a subject specific folder.

Steve