Re: Difference in two images

Posted by Herbie-3 on Feb 02, 2014; 3:28pm
URL: http://imagej.273.s1.nabble.com/Difference-in-two-images-tp5006370p5006371.html

Prashant Wasnik,

if you wish to obtain the mathematical difference of the two images then use
        Process > Image Calculator...

First convert both images to 32 bit, then use "Image Calculator..." with
"Operation" either "Difference" or "Subtract". The former mode results
in an image containing the absolute value of the difference of both
images. Finally, you may convert the result image to 8 bit.

HTH

Herbie

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On 02.02.14 08:37, Prashant Wasnik wrote:
> Respected Sir,
> I am working on lipids and want to determine the difference among these
> images. I have tried to measure the pixel intensities, particle analysis,
> GLCM after removing Gausian blur at 25 sigma but unfortunately I not
> getting the appropriate difference.  I am attaching two images for your
> kind reference. Kindly suggest the methodology.
> Thanking You
> With Regards

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