How to quantify a regular arrangement on my images?

Posted by lechristophe on Feb 05, 2014; 4:11pm
URL: http://imagej.273.s1.nabble.com/How-to-quantify-a-regular-arrangement-on-my-images-tp5006427.html

Dear ImageJists,

I have images from immunocytochemistry (proteins labeled with an antibody
in a cell culture), and I'd like to quantify a striking feature: one of the
proteins is periodically organized (see Image1.tif), whereas the other
seems to be more randomly clusterized (see Image2.tif). The exemple images
are a crop of the whole image, but basically this is the pattern for the
whole labeled structure.

I'd like to analyze and quantify how "band-like" is the pattern, i.e. a
number that would be a measure of the labeling disorder, from completely
periodic to completely random. I don't know how to do it. I tried the
OrientationJ and Directionality plugins, but they seem to find orientations
in both cases, and I'm more interested in the intensity of the organization
than in the directions of the pattern.

If you have any advice on how to do this, that would be great!

Thanks for your help,

Christophe

P.S. In case the attachments are stripped, you can find the images here:
Image1.tif: http://www.cleterrier.net/up/Image1.tif
Image2.tif: http://www.cleterrier.net/up/Image2.tif

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