Posted by
Herbie-3 on
Feb 05, 2014; 5:24pm
URL: http://imagej.273.s1.nabble.com/How-to-quantify-a-regular-arrangement-on-my-images-tp5006427p5006429.html
Christophe.
that's a nice one for Fourier-Analysis!
Apply "FFT" to your images which gives you the log of the Power Spectra
as 8bit images. You may now extract features from these.
One of such features could be the integral along straight lines through
the spectral origin as a function of the angle. Another could be the
dual function, i.e. the integral on circles around the spectral origin
as a function of their radii. There are PlugIns for both features. More
precision is obtained if you use 32bit spectra.
HTH
Herbie
::::::::::::::::::::::::::::::::::::::::::::::
On 05.02.14 17:11, Christophe Leterrier wrote:
> Dear ImageJists,
>
> I have images from immunocytochemistry (proteins labeled with an antibody
> in a cell culture), and I'd like to quantify a striking feature: one of the
> proteins is periodically organized (see Image1.tif), whereas the other
> seems to be more randomly clusterized (see Image2.tif). The exemple images
> are a crop of the whole image, but basically this is the pattern for the
> whole labeled structure.
>
> I'd like to analyze and quantify how "band-like" is the pattern, i.e. a
> number that would be a measure of the labeling disorder, from completely
> periodic to completely random. I don't know how to do it. I tried the
> OrientationJ and Directionality plugins, but they seem to find orientations
> in both cases, and I'm more interested in the intensity of the organization
> than in the directions of the pattern.
>
> If you have any advice on how to do this, that would be great!
>
> Thanks for your help,
>
> Christophe
>
> P.S. In case the attachments are stripped, you can find the images here:
> Image1.tif:
http://www.cleterrier.net/up/Image1.tif> Image2.tif:
http://www.cleterrier.net/up/Image2.tif>
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