Posted by
Theresa Swayne on
Feb 07, 2014; 6:38pm
URL: http://imagej.273.s1.nabble.com/To-make-a-image-from-several-images-tp5006451p5006454.html
Hello,
I'm not sure if I understand what you want, but here are some possibilities:
If you want to merge the images as multiple channels:
1. Use Image > Color > Channels Tool, click More >> Merge Channels… and select your images (don't worry that it says red/green/blue -- you will change it later) and check the box for Create Composite.
2. Restore each image to grayscale: in Channels Tool, choose Color mode, check Channel 1, then More >> Grays. Do the same for each channel. Then More >> Convert to RGB to get a single image.
If you want a projection (e.g. with each pixel replaced by the maximum, minimum, or average of that position in all images), make a stack of the images and then Image > Stacks > Z Project.
If you want a montage (multiple panels), make a stack and Image > Stacks > Make Montage.
Hope one of these ideas is helpful.
Theresa
On Feb 7, 2014, at 12:25 PM, JoeML <
[hidden email]> wrote:
>
> I have a doubt about to create a only image from two or three images. I
> tried doing a stack but when i try to "make composite" it doesnt work or
> modifies the original grayscale. Anybody know some way to make a only image
> from several images (2 or 3) and keep those images in grayscale.
>
>
------------------------------------
Theresa Swayne, Ph.D.
Associate Research Scientist
Manager, Confocal and Specialized Microscopy Shared Resource
Herbert Irving Comprehensive Cancer Center, Columbia University
1130 Saint Nicholas Ave, 222A
New York, NY 10032
212-851-4613
[hidden email]
http://hiccc.columbia.edu/research/sharedresources/confocal--
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