Re: Advice segmenting a short video

Posted by Pedro J CamelloDr Pedro J Camello on Mar 01, 2014; 3:04pm
URL: http://imagej.273.s1.nabble.com/Advice-segmenting-a-short-video-tp5006685p5006728.html

Hi Charles,

I´ve been unable to reproduce the behaviour you describe. The images are very small (abount 100 x 30 pixels) so that pixels are perfectly differentiated. I have tried to change from 8 to 16 and 32 bits but I see no difference. I have even made a Plot Profile for a vertical line in my original and in 16 and back-to 8 bit images and the plots are the same.
After the conversion I still have the same problem with automated segmentation/thresholding (in fact, in my first attempts I used 32 bit images)

Thanks for your reply

Pedro

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