Isolating an object in image

Posted by Sophie Lorent on
URL: http://imagej.273.s1.nabble.com/Isolating-an-object-in-image-tp5006745.html

Hi,

I am trying to isolate unicellular algae from an image. I have tried to use filters, thresholds, math, gamma …

But the sometimes the inside of my cells are as nearly the same light  colour as the background. When this happens on the border of the cell, this part will be cut off the cell without being able to save it by “filling the holes” like I do it when these light parts occur in the middle on the cell. (See attachment figure 1 and 2)

Is there something that can make can make kind on join two edges? (See figures 1 and 2). Or if someone has a good idea on how I could solve this problem.

Cheers,

Sophie

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