Quantifying Myotubes and Nuclei Count

Posted by Mevan Siriwardane on
URL: http://imagej.273.s1.nabble.com/Quantifying-Myotubes-and-Nuclei-Count-tp5006755.html

Hi,

I would like to use imageJ to count the number of elongated myotubes in a 40 or 60X magnification.  Additionally, we would also like to automate the cell counting of nuclei within each myotube (a myotube is formed when myoblasts fuse together creating elongated structures of 2 or more multi nuclei.  

In a 60X FOV, we would like to use imagej for counting the number of myotubes, quantifying the number of multi nuclei in each myotube and counting the total number of nuclei.  Are there any plugins that any of you would recommend for this application?

I would be happy to attach a representative image of a 60X FOV with myotubes for analysis.

Look forward to the feedback.


Thank you,

Mevan Siriwardane

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