Re: Count foci within each cell

Posted by Aryeh Weiss on Mar 04, 2014; 10:53am
URL: http://imagej.273.s1.nabble.com/Count-foci-within-each-cell-tp5006766p5006767.html

On 3/4/14, 12:10 PM, chin wrote:
> Hi,
>
> I would like to count foci within each cell. Is there a way or macro to
> perform the approach?
> please have a look at the image.
> <http://imagej.1557.x6.nabble.com/file/n5006766/C1foci..jpg>
> I tried to use threshold first but seem not obtain a good result. Any advice
> would be appreciated.
>
Try Process>Find Maxima...
A noise tolerance of 20 looks ok.
You can also find the borders of the cells by thresholding the image,
and then discarding the small objects and closing the result.
Gabriel Landini's BinaryFilterReconstruct is a good way to delete the
foci while leaving the borders.

--aryeh

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--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
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