help for an image j newbie please

Posted by k k on Mar 05, 2014; 3:18am
URL: http://imagej.273.s1.nabble.com/help-for-an-image-j-newbie-please-tp5006774.html

hello
i am a grad student and have recently discovered that i need to calculate
total surface area as well as the surface area of the right and left
ventricle from a cross-sectional picture of a pig heart.  I am hoping image
j can help me out but have never used the software before.  I would like
the program to outline the outer and inner edges of the heart walls
automatically and then calculate the surface area of what has been
outlined. In the cross section image the ventricle chambers are "holes"
which I do not want included in the area calculation.  I tried following
the sample method of using the variegated leaf found here (
http://rsb.info.nih.gov/ij/docs/pdfs/examples.pdf), but I am having a hard
time separating the heart from the background in the image.  I will
ultimately need to perform these calculations for 150 images, so I am
hoping to automate this process as much as possible.

thanks for any assistance.

kathy

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