Re: Measuring protein expression intensity

Posted by Gabriel Landini on Mar 05, 2014; 9:14am
URL: http://imagej.273.s1.nabble.com/Measuring-protein-expression-intensity-tp5006771p5006779.html

On Tuesday 04 Mar 2014 13:59:34 Demilux wrote:
> I'm aware that quantifying is relative when it comes to bright field
> microscopy, but I still want to extract some relative numbers from my
> photographs. I am to "measure" the intensity of the expression of a protein
> in certain ROIs. It was suggested to me to use histograms and extract values
> from them as a means of relative quantification.

It is not "relative", it is "unreliable". That's different.
So no matter how reproducible the imaging part is, the optical density of the
ROIs is not a true representation of the protein expression.

Please see the mail archives in relation to quantifying immunohistochemistry.


Regards

Gabriel

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