Re: Count foci within each cell

Posted by chin on Mar 06, 2014; 12:57am
URL: http://imagej.273.s1.nabble.com/Count-foci-within-each-cell-tp5006766p5006795.html

Thanks both aryeh and Jan's suggestion.

I could almost figure out and learn the steps from it.
However, i am still  confused a little bit about the those folloing steps:

From aryeh's method:
//You can also find the borders of the cells by thresholding the image,
and then discarding the small objects and closing the result. //

From Jan's method:
//run the "Watershed Irregular Features" macro from the BioVoxxel Toolbox
(e.g. with an erosion cycle of 8 or manually close areas of cells according
to the original image if not possible differently)

How could I "manually close areas of cells according to the original image if not possible differently"

Could you explain it more detail?

Thanks again.

Best regard,

chin