Re: Stereologically count triple labelled nuclei in 3D in ImageJ

Posted by Jeremy Adler on Mar 07, 2014; 12:36pm
URL: http://imagej.273.s1.nabble.com/Stereologically-count-triple-labelled-nuclei-in-3D-in-ImageJ-tp5006801p5006818.html

I am not sure that rejecting nuclei that touch either the upper or lower planes is the correct selection criteria.
This produces a bias against large nuclei or elongated nuclei.
It is better to exclude nuclei that cut one of the outer planes, but include those that cut the other plane.

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Federico Luzzati
Sent: den 6 mars 2014 14:38
To: [hidden email]
Subject: Stereologically count triple labelled nuclei in 3D in ImageJ

Dear list,
I would like to count nuclei labelled with three markers in a histological section acquired with the confocal microscope. In this analysis I would like to obtain the total number of objectes labelled for each marker and of those that are co-labelled. In addition I would like to exclude  all the nuclei that contact the upper (or lower) confocal plane in order to have a stereological estimate of the particles in the analyzed volume.

Any suggestion to perform an automatic analysis (maybe with the 3D object counter and Coloc plugins?) will be greately appreciated!

Kinds regards

Federico Luzzati

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Federico Luzzati, PhD
Researcher
Dept. Life Sciences and Systems Biology
University of Turin
Via Accademia Albertina, 13
10123 Torino
Neuroscience Institute Cavalieri Ottolenghi (NICO) Regione Gonzole, 10
10043 Orbassano (Torino)
ITALY
tel. [phone]/ -6631

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