Re: Image Stack Color Analysis

Posted by Michael Schmid on Mar 10, 2014; 1:49pm
URL: http://imagej.273.s1.nabble.com/Image-Stack-Color-Analysis-tp5006839p5006850.html

Hi A.,

probably the method of choice is creating a composite image from the stack:
Image>Color>Make Composite.

Then use the 'Channels' window to set the colors of the channels, etc.
See
  http://rsb.info.nih.gov/ij/docs/guide/146-28.html#sub:Channels...[Z]

For the concentrations, one option is to select the area and use the Roi Managers 'Multi Measure' (in the 'More>>' menu). It will only give you the channel number, not the channel name.

If you want to have more functionality, there are macros or plugins around, or you can write your own one. A simple starting point might be
  http://rsb.info.nih.gov/ij/macros/MeasureStack.txt

Michael
________________________________________________________________
On Mar 10, 2014, at 04:10, ataulf wrote:

> Hello,
>
> I have a few images of the same rock (a stack) with each of them revealing
> the presence of a certain element in a different color. So one of them will
> show bright green when Silicon is present, another may show dark red where
> Calcium is present, etc.
>
> Is there any way to overlay these and be able to tell what is the
> concentration of all the minerals at a given point on the rock?
>
> Thank you for your time.
>
> Best regards,
> A. Taulf

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