Area fraction within multiple ROIs

Posted by lindaP on
URL: http://imagej.273.s1.nabble.com/Area-fraction-within-multiple-ROIs-tp5006915.html

Hi there,

I'm trying to figure out how to conduct the following particle analysis in the most efficient way:

On a large number of similar images , I'd like to measure:

1) individual areas of all particles (in yellow in the example image)
2) area fraction of all particles within a larger region of interest (in red in the example image). In some images, I have only one ROI, in other images more then one ROIs.

I'd like to write a little macro when one would manually draw the particles and ROIs around these particles and then the macro would output a file with particle sizes and another file with the fraction area (% fraction area of particles from the corresponding ROI area).
It would be ideal to be able to add results from subsequent images to the same output files later on.

Thanks for your suggestions!

Linda