Re: Nuclei segmentation
Posted by
dscho on
Mar 17, 2014; 3:56pm
URL: http://imagej.273.s1.nabble.com/Nuclei-segmentation-tp5006953p5006954.html
Hi Chin,
On Mon, 17 Mar 2014, chin wrote:
> Are there better ways to segment those various types of nuclei in a image?
> I tried some threshold methods to segment then use watershed to split
> connect nuclei. But it seems not be segmented completely by using those
> methods.
You might need to apply some background subtraction with a real large
diameter for the threshold, and then use the "Watershed Irregular
Structures" command in the BioVoxxel Toolbox:
http://fiji.sc/BioVoxxel_Toolbox#Watershed_Irregular_StructuresCiao,
Johannes
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