Re: Nuclei segmentation

Posted by Adam Hughes on Mar 17, 2014; 4:01pm
URL: http://imagej.273.s1.nabble.com/Nuclei-segmentation-tp5006953p5006955.html

What about supervised segmentation with weka or ilastik?
On Mar 17, 2014 11:57 AM, "Johannes Schindelin" <[hidden email]>
wrote:

> Hi Chin,
>
> On Mon, 17 Mar 2014, chin wrote:
>
> > Are there better ways to segment those various types of nuclei in a
> image?
> > I tried some threshold methods to segment then use watershed to split
> > connect nuclei. But it seems not be segmented completely by using those
> > methods.
>
> You might need to apply some background subtraction with a real large
> diameter for the threshold, and then use the "Watershed Irregular
> Structures" command in the BioVoxxel Toolbox:
>
> http://fiji.sc/BioVoxxel_Toolbox#Watershed_Irregular_Structures
>
> Ciao,
> Johannes
>
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