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Re: Nuclei segmentation

Posted by William Menegas on Mar 17, 2014; 4:11pm
URL: http://imagej.273.s1.nabble.com/Nuclei-segmentation-tp5006953p5006956.html

Those tools are both great as long as input size isn't too large!
On Mar 17, 2014 12:03 PM, "Adam Hughes" <[hidden email]> wrote:

> What about supervised segmentation with weka or ilastik?
> On Mar 17, 2014 11:57 AM, "Johannes Schindelin" <
> [hidden email]>
> wrote:
>
> > Hi Chin,
> >
> > On Mon, 17 Mar 2014, chin wrote:
> >
> > > Are there better ways to segment those various types of nuclei in a
> > image?
> > > I tried some threshold methods to segment then use watershed to split
> > > connect nuclei. But it seems not be segmented completely by using those
> > > methods.
> >
> > You might need to apply some background subtraction with a real large
> > diameter for the threshold, and then use the "Watershed Irregular
> > Structures" command in the BioVoxxel Toolbox:
> >
> > http://fiji.sc/BioVoxxel_Toolbox#Watershed_Irregular_Structures
> >
> > Ciao,
> > Johannes
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
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>

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