Re: Finding Multiple Peaks in a Plot Profile

Posted by George Patterson on Mar 17, 2014; 11:28pm
URL: http://imagej.273.s1.nabble.com/Finding-Multiple-Peaks-in-a-Plot-Profile-tp5006959p5006960.html

You might try the Beanshell script here (http://fiji.sc/Find_Peaks) in FIJI.
George


On Mon, Mar 17, 2014 at 6:51 PM, Burni <[hidden email]> wrote:

> Hello,
>
> this is my first post in this forum and I'm not very experienced with
> ImageJ.
> I've been looking for help with a particular problem where I'll probably be
> using the Plot Profile of an image.
> The following is an example of the raw image I'll be using:
>
> <
> http://imagej.1557.x6.nabble.com/file/n5006959/HITRAP_IH_EA_2011.09.21_21.05.42.752_ImgID_245.png
> >
>
> This one is basicly the same, but I adjusted the contrast a little so you
> can better see what's happening.
>
> <
> http://imagej.1557.x6.nabble.com/file/n5006959/HITRAP_IH_EA_2011.09.21_21.05.42.752_ImgID_245_Edit.png
> >
>
> When I generate the Plot Profile of that image using control+alt+k (I
> specifically need this one, not the one using control+k), I get this:
>
> <http://imagej.1557.x6.nabble.com/file/n5006959/PlotProfile.png>
>
> What I'm finally looking for is a way to reliable find the positions (or
> more specifically the x-coordinates) of these peaks in whatever Plot
> Profile
> I generate. There is a different number and height of peaks depending on
> each picture.
>
> <http://imagej.1557.x6.nabble.com/file/n5006959/PlotProfile_Peaks.png>
>
> My first guess was to use the fitting tool of ImageJ, but it seems to be
> too
> limited to use it for this task.
> I'm not even sure if fitting would be necessary at all.
> I would be very thankful for any suggestions on how to go about this
> problem.
> I uploaded the raw image below.
>
> Thanks in advance to anyone, who might help me.
>
> HITRAP_IH_EA_2011.09.21_21.05.42.752_ImgID_245.png
> <
> http://imagej.1557.x6.nabble.com/file/n5006959/HITRAP_IH_EA_2011.09.21_21.05.42.752_ImgID_245.png
> >
>
>
>
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>
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