Re: Nuclei segmentation

Posted by chin on Mar 20, 2014; 6:28am
URL: http://imagej.273.s1.nabble.com/Nuclei-segmentation-tp5006953p5007007.html

Hi Michael,

Thank you for the Adjustable Watershed. It does pretty well on segmentation when some non-circular
objects need not to be segmented. I tried the "Tolerance" of 2.3 and obtained the satisfied segmentation.
There are only four pairs of nuclei contact side by side.
 
This plugin is really useful not only for me but for those who want to use more flexible watershed to
segment their objects.

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Hi Kees,

Your macro gives me another approach to use Michael's plugin. I didn't mention about the unit of original
image is um. The macro didn't show the "Action Required: Separate cells" by using the perim>220. So I
change the value to 90, the result looks great. Thank you for your assistance.

Again, Thank both Michael and Kees.

best,
chin