Re: TrackMate: LAP Tracker not enough memory

Posted by Jean-Yves Tinevez on
URL: http://imagej.273.s1.nabble.com/TrackMate-LAP-Tracker-not-enough-memory-tp5006980p5007008.html

Hi Karen.
>
> Hi Jean,

Jean-Yves :)

> Thanks for the explanation on how LAP tracker do the linking of each spot.
> However I need the merging events as my cells sort of "touch" each other
> while moving. I also have a long video (10min, 1fps) with many cells
> (300-400 per frame)

This is the kind of use-case that stretch TrackMate to its limits. For now.
If investigating another solution (ICY, Imaris, u-track, …) is impossible, a workaround would be to forbid merging event (having only linear tracks) and correcting the problems with touching cells manually. But I guess with a movie of that size this is impracticable.
>
> Since its the spots in the matrix that the LAP tracker computes, then
> lowering the number of pixels and image size would not help in the memory
> problem of tracking, am I right?

You are right.

best
jy


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Jean-Yves Tinevez
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