Re: Nuclei segmentation

Posted by gankaku on Mar 20, 2014; 10:37am
URL: http://imagej.273.s1.nabble.com/Nuclei-segmentation-tp5006953p5007018.html

Hi all,

I also tried the adjustable watershed plugin which is really good and I
think it would be a good idea to integrate this in one of the following new
versions of ImageJ or distribute it by default with the Fiji package.
Very nice and useful tool (actually makes the 'watershed irregular
features' macro from the biovoxxel toolbox kind of obsolete, but I still
haven't compared the performances of both in different images).

cheers,
Jan


2014-03-20 7:28 GMT+01:00 chin <[hidden email]>:

> Hi Michael,
>
> Thank you for the Adjustable Watershed. It does pretty well on segmentation
> when some non-circular
> objects need not to be segmented. I tried the "Tolerance" of 2.3 and
> obtained the satisfied segmentation.
> There are only four pairs of nuclei contact side by side.
>
> This plugin is really useful not only for me but for those who want to use
> more flexible watershed to
> segment their objects.
>
> -----------------------
>
> Hi Kees,
>
> Your macro gives me another approach to use Michael's plugin. I didn't
> mention about the unit of original
> image is um. The macro didn't show the "Action Required: Separate cells" by
> using the perim>220. So I
> change the value to 90, the result looks great. Thank you for your
> assistance.
>
> Again, Thank both Michael and Kees.
>
> best,
> chin
>
>
>
>
>
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> Sent from the ImageJ mailing list archive at Nabble.com.
>
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