Image Calculator across many image sets

Posted by mfloyd78 on Mar 31, 2014; 8:18pm
URL: http://imagej.273.s1.nabble.com/Image-Calculator-across-many-image-sets-tp5007157.html

Hello everyone,  I have a problem that I am hoping someone can help me work out. I am trying to figure out how to use a set Image Calculator function across multiple sets of images without manually doing it each time. Each image set contains a picture of DAPI stained nuclei and an image of cytoplasm stained cells. I am subtracting the images of the DAPI stain from the images of the cells create objects with the total area of the cell minus the nuclei. Right now I am going through and performing this function with each individual set and was wondering if there is a way to set up a Macro to automate this to be done with multiple sets all at once. Any help would be greatly appreciated. Thanks!