Re: Fiji - Math

Posted by LIM Soon Yew John (IMB) on Apr 08, 2014; 3:02am
URL: http://imagej.273.s1.nabble.com/Fiji-Math-tp5007235p5007236.html

Hi Haimon,

You convert your image to 32 bit using menu command Image>Type>32-bit before you applied your calculation. You can then scale it back to 8 bit or 16 bit at a later stage.

Best Regards,
John
________________________________________
From: ImageJ Interest Group [[hidden email]] On Behalf Of Haimon Alves [[hidden email]]
Sent: Tuesday, April 08, 2014 10:45 AM
To: [hidden email]
Subject: Fiji - Math

I need to perform some mathematical calculations on
grayscale images. However, each multiplication results in
a saturated image (everything below 255 or 65535 eventually
goes to those numbers).

So, is there anyway possible to avoid this? Like, do the math,
the software saves the numbers - even if the image does not
make any sense at first, and then, when everything is done,
those new values are scaled back to 0-255 or 0-65535?!

Thank you for your help

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*Haimon*

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