Re: Fiji - Math

Posted by Haimon Alves on Apr 08, 2014; 3:12am
URL: http://imagej.273.s1.nabble.com/Fiji-Math-tp5007235p5007237.html

Hi John,

thanks for your help. one question though: wouldn't that be the same
problem, if
the values goes over the maximum of a 32 bit? Because if any value goes
beyond
of what I need, it will not help.

what I wanted was not to worry about those limits. I just wanted to make
those
calculations and, after those changes, I would scale to an actual image.


2014-04-08 0:02 GMT-03:00 LIM Soon Yew John (IMB) <
[hidden email]>:

> Hi Haimon,
>
> You convert your image to 32 bit using menu command Image>Type>32-bit
> before you applied your calculation. You can then scale it back to 8 bit or
> 16 bit at a later stage.
>
> Best Regards,
> John
> ________________________________________
> From: ImageJ Interest Group [[hidden email]] On Behalf Of Haimon
> Alves [[hidden email]]
> Sent: Tuesday, April 08, 2014 10:45 AM
> To: [hidden email]
> Subject: Fiji - Math
>
> I need to perform some mathematical calculations on
> grayscale images. However, each multiplication results in
> a saturated image (everything below 255 or 65535 eventually
> goes to those numbers).
>
> So, is there anyway possible to avoid this? Like, do the math,
> the software saves the numbers - even if the image does not
> make any sense at first, and then, when everything is done,
> those new values are scaled back to 0-255 or 0-65535?!
>
> Thank you for your help
>
> --
>
> *Haimon*
>
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*Haimon*

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