Posted by
PEARSON Matthew on
Apr 18, 2014; 8:16am
URL: http://imagej.273.s1.nabble.com/Image-stitching-advice-tp5007349.html
Hi all,
Quite often in our facility we capture "large images", stitching
multiple fields of view together to produce an image of a larger
area. This is done on a confocal microscope and the cells we're
looking at are motile hence the need to tile as they often move out of
the field of view of the objective lens. In terms of capturing the
data there is an option for the capture software to stitch or not. If
we don't tell the software to stitch then we're relying on the
accuracy of the stage to join the tiles. If we stitch we use
something like 15% overlap. The stitching is not always accurate
though and i understand that cells moving around the field could be
problematic from a stitching point of view.
So basically i'm wondering if we do not stitch whether we can look to
other applications to carry out the stitching or at least try them
out. Does anyone have any recommendations on ImageJ/FIJI plugins to
use to stitch tiles from confocal time lapse data? There seems to be
quite a few plugins related to this. I've tried StackReg and this
does work on the data but it seems quite slow and we're capturing
quite large data sets at different xy stage positions.
Many thanks,
Matt
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