Re: Selecting correct threshold algorithm

Posted by tmurp002 on Apr 21, 2014; 9:52pm
URL: http://imagej.273.s1.nabble.com/Selecting-correct-threshold-algorithm-tp5007356p5007380.html

Thank you gankaku, those BioVoxxel tools really helped. Threshold check seems to confirm that the mean threshold is a good way to go. MoLim and DiLim do look very accurate, and in fact give similar/nearly identical results to the mean algorithm (which makes sense) but require more effort to use since they don't work on stacks, and I'm a bit wary of changing my images to 8-bit prior to thresholding. So I think I'll stick with the mean threshold for now.

As to your first point, I've considered 3D analysis (and I'll definitely check out that link), although it has proven too complicated to implement thus far. Perhaps I was simply using the wrong tools before.

Thanks again!