How to turn overlays into ROI's

Posted by PEARSON Matthew on Apr 23, 2014; 6:53pm
URL: http://imagej.273.s1.nabble.com/How-to-turn-overlays-into-ROI-s-tp5007404.html

Hi all,

I'd like to be able to isolate areas of a fluorescence image to work on and there a few points of interest.  As ImageJ only supports one selection/ROI at a time could i use the overlay brush tool to paint over various points (if i use a large ish brush diameter) then convert these to ROI's or at least use the ROI manager to select each overlay in turn then make it an ROI-analyse it then move on to the next.  I tried Image>Overlay>To ROI Manager but this doesn't seem to treat each overlay the same as it would a regular selection.  I'd like to threshold based on the intensity under each overlay therefore it must become an ROI first, the overlay is purely to select parts of the image for further analysis.

Many thanks,

Matt



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