Problem regarding cell counting

Posted by SARAH ROSE STERLACE on Apr 24, 2014; 4:54pm
URL: http://imagej.273.s1.nabble.com/Problem-regarding-cell-counting-tp5007416.html

Hello,
I am having trouble how to figure out a way to automatically count the GFP
stain without counting the background. I have been using the ITCN plugin,
but I cannot figure out how to have it just count the circles.  Any help
would be greatly appreciated.
I have been inverting the image, setting it to 8-bit and enhancing the
contrast by 3% to show the cells stained deeper in the tissue better to see
if that would help.  (i have not attached that image because it exceeds the
size for the email) I am attaching an original image. I have about 100
images to count, so if I could figure out how to automate the counting that
would be awesome!

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Sarah R Sterlace, C. Phil

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