Posted by
carlo bianco on
Apr 24, 2014; 10:09pm
URL: http://imagej.273.s1.nabble.com/Problem-regarding-cell-counting-tp5007416p5007426.html
hi
try with color segmentation plugin
http://bigwww.epfl.ch/sage/soft/colorsegmentation/manually. than, if you have the area you can divide for the mena area of a positive signal (cell or nucleus)
to obtain the number of positive signals.
But maybe is better to acquire photos avoiding
central field overexpasure, or correct it.
hope this is useful
best wishes
carlo
bologna university
Il Giovedì 24 Aprile 2014 23:38, SARAH ROSE STERLACE <
[hidden email]> ha scritto:
Hello,
I am having trouble how to figure out a way to automatically count the GFP
stain without counting the background. I have been using the ITCN plugin,
but I cannot figure out how to have it just count the circles. Any help
would be greatly appreciated.
I have been inverting the image, setting it to 8-bit and enhancing the
contrast by 3% to show the cells stained deeper in the tissue better to see
if that would help. (i have not attached that image because it exceeds the
size for the email) I am attaching an original image. I have about 100
images to count, so if I could figure out how to automate the counting that
would be awesome!
--
Sarah R Sterlace, C. Phil
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