Re: Quantification problem: more values despite weaker band?

Posted by Rena2014 on Apr 28, 2014; 1:40pm
URL: http://imagej.273.s1.nabble.com/Quantification-problem-more-values-despite-weaker-band-tp5007430p5007470.html

Hi,

Thanks already for your comments on my quantification problem!
So far I followed a protocol from the University of Missouri (http://support.dalton.missouri.edu/index.php/wiki/Public:Quantifying_Color_Intensity/).
As recommended, I checked the "true intensities" and in some of my images I see high values for the black bands and low values for the white background (if the image is not inverted). So, clearly here is something inverted, right?