http://imagej.273.s1.nabble.com/plugin-macro-to-automatically-find-and-measure-peak-width-tp5007535p5007537.html
tolerance. This operation provides the point selections and you may get
the coordinates simply by e.g. using the menu command "Measure". No big
Fitting a Gaussian is a different matter. I guess a 2D-Gaussian is
but maybe you will find a plugin at the IJ-Website. Did you try?
> Hello, I'm writing this email on behalf of a colleague who is not
> part of the ImageJ email listserver. This person is wondering if
> there is an existing macro or plugin that can automatically find and
> measure the widths of peaks in an image of point-sources of light.
> For example, his images might look like this one:
>
>
http://fiji.sc/images/2/26/Disc60xwithoutoptovar.gif>
> The macro/plugin would ideally find each source of light (in this
> case a pinhole in a Nipkow disk), fit a Gaussian distribution to this
> point-source along x and y line profiles drawn through the point,
> then extract the standard deviation / FWHM of both fits, and finally
> log the results into a text file.
>
> So far I've been able to construct a simple macro that finds the
> pinholes using the analyze particles function (searching for
> centroids) and limiting to a threshold, and then using the xy
> coordinates of the centroids to act as positions for drawing line
> profiles through. I think the next step would be to use ImageJ's
> curve-fitting algorithm to get the fitting data.
>
> Again, not want to re-invent the wheel here, so just wanted to check
> if there's something out there that can already do this. Would the
> MetroloJ plugin work in this situation, or does that only work with
> single points of light in an image (like a fluorescent bead in a
> micrograph)? My colleague would also ideally have the ability to do
> this calculation rapidly on the fly as the image is acquired (with
> maybe a few seconds delay maximum). Maybe MicroManager could do this
> through it's scripting functions?
>
> Any help here is much appreciated!
>
>
> Sincerely,
>
>
> John Oreopoulos Staff Scientist Spectral Applied Research Inc. A
> Division of Andor Technology Richmond Hill, Ontario Canada
> www.spectral.ca
>
>
>
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http://imagej.nih.gov/ij/list.html>