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Counting bubbles

Posted by YuriGagarine on May 06, 2014; 12:07pm
URL: http://imagej.273.s1.nabble.com/Counting-bubbles-tp5007594.html

Hi all,
I am posting because I am stucked for weeks. I have a macro to do which have to count bubbles, to have their distances from the bottom and their radius and the %Area of bubbles.

What I have done so far was :
- Capture two images, one lighted to allow the user to select the bottom and one without light to be easier to analyse (this step is done in order to be able to calculate the distance of each bubble from the bottom and also calculate the %Area)

- Compute an automatic thresholding that work well (I used the default one, I think it is the ISODATA algorithm)

- Compute the "Ultimate Points" after the thresholding and recover the results in order to draw circles on the original image and see if it worked well.

Because of fissures in the glass and bubbles that are out of focus it is hard to have appropriate results.

What I am asking for are proposals for my project, I am running low of ideas now.

Here are the images :
lighted image
Image that I analyse
Zoom in the image after the processing

Thanks in advance and sorry for my english.