Posted by
Herbie-3 on
May 06, 2014; 1:42pm
URL: http://imagej.273.s1.nabble.com/Counting-bubbles-tp5007594p5007599.html
Yuri,
no help with your problem...
...if this
> Because of fissures in the glass and bubbles that are out of focus it
> is hard to have appropriate results.
is the cause.
-- Image acquisition contributes most importantly to the quality of
automatic image analysis.
-- Most often you cannot compensate for bad conditions in image
acquisition by post ho image processing.
Your experimental conditions support these statements.
The problem with out-of-focus bubbles can perhaps partially be remedied
but the glass defects will disturb as long as you work with this kind of
illumination.
The very dark structures are plants I guess. Don't they disturb as well?
Best regards
Herbie
::::::::::::::::::::::::::::::::::::::
On 06.05.14 14:07, YuriGagarine wrote:
> Hi all,
> I am posting because I am stucked for weeks. I have a macro to do which have
> to count bubbles, to have their distances from the bottom and their radius
> and the %Area of bubbles.
>
> What I have done so far was :
> - Capture two images, one lighted to allow the user to select the bottom and
> one without light to be easier to analyse (this step is done in order to be
> able to calculate the distance of each bubble from the bottom and also
> calculate the %Area)
>
> - Compute an automatic thresholding that work well (I used the default one,
> I think it is the ISODATA algorithm)
>
> - Compute the "Ultimate Points" after the thresholding and recover the
> results in order to draw circles on the original image and see if it worked
> well.
>
> Because of fissures in the glass and bubbles that are out of focus it is
> hard to have appropriate results.
>
> What I am asking for are proposals for my project, I am running low of ideas
> now.
>
> Here are the images :
> <
http://imagej.1557.x6.nabble.com/file/n5007594/3-eclaire.jpg>
> <
http://imagej.1557.x6.nabble.com/file/n5007594/333333.jpg>
> <
http://imagej.1557.x6.nabble.com/file/n5007594/Sans_titre.png>
>
> Thanks in advance and sorry for my english.
>
>
>
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