Posted by
Mike Holloway on
May 07, 2014; 5:52pm
URL: http://imagej.273.s1.nabble.com/Using-color-threshold-and-analyze-particles-to-compare-colocalization-tp5007617.html
I'm attempting to compare degrees of colocalization in zebrafish motor
axons. I've been researching ways to measure colocalization for months now
and I'm still uncertain of the best way to procede. Read the Coloc2
instructions and accompanying guides, but that software seems designed to
accurately detect colocalization rather than allow comparison of degrees of
colocalization between many control and experimental samples. If I'm wrong
about that please correct me, but I can't see how the coefficients can be
used in this way, and have failed to find any paper in which they are used
that way. What I've settled on is masking axons, making a max intensity Z
projection image, isolating a narrow band of colocalized color (yellow) with
color threshold set to default, and using analyze particles to find the
percent of the total area that the colocalized puncta take up. So far I've
found no difference between my experimental and control which seems to be
telling me that either this method is kosher, or I'm messing up big time.
As I said, if there's something in the ImageJ/Fiji docs that address this
I'm not picking up on it. Been over them several times. If anyone has
suggestions, or assurances, please let me know.
Thanks,
Mike Holloway
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