Deleting an ROI by clicking on it

Posted by Jmuller on May 09, 2014; 6:52pm
URL: http://imagej.273.s1.nabble.com/Deleting-an-ROI-by-clicking-on-it-tp5007671.html

Hi,

I am interested in a macro or plugin that will either let me record an ROI ID number or delete it by clicking on it. Is any one aware of a tool like this that might currently exist?

The reason I need this functionality is that I am doing some semi automated image analysis by making a mask of immunofluorescently labeled cells and then measuring the intensity for each cell in each channel from the ROI manager. I generally have 10-15 conditions and take 9*9 tile images that contain 300+ cells.

So far I can get about 85-90% accuracy of correctly outlining the cell and excluding cells that are touching by using a combination of circularity, water shedding and size exclusion. However, it still requires manually going through each image and then writing down the bad ROI and then I have a python script that will delete then from the results file. If I could record the ROI just by clicking on them in imageJ this would really speed up my image analysis and I think provide a sufficient level of automation with a high level of accuracy.  

Any help would be greatly appreciated,

Thanks,

James Muller