Login  Register

Re: Pixel Normalisation - Need help

Posted by Michael Schmid on May 13, 2014; 1:24pm
URL: http://imagej.273.s1.nabble.com/Pixel-Normalisation-Need-help-tp5007712p5007714.html

Hi anonymous,

is Analyze>Set Scale what you want?

http://rsb.info.nih.gov/ij/docs/guide/146-30.html#toc-Subsection-30.8

The scale applies to a whole stack, i.e., all images (slices) in the stack will have the same number of pixels per mm (or per µm, or whatever your sizes are).

Michael
________________________________________________________________
On May 13, 2014, at 13:38, bmm7 wrote:

> Dear All,
>
> I am trying to measure the lengths of objects along a path of a known
> distance. Each image is taken from a slightly different location (ie the
> pixels are different). There is a stack of images and I am forced to divide
> the "distance in pixels" by "known distance" for each image to normalise
> between the samples. It is very energy and time consuming. Is there an
> alternative method that i have overlooked? ImageJ should be able to do that
> automatically right?
>
> Thanks in advance,

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html