Posted by
PEARSON Matthew on
May 19, 2014; 11:14am
URL: http://imagej.273.s1.nabble.com/Segmentation-of-confluent-cells-tp5007785.html
Hi all,
I've been asked whether its possible to measure the area of individual
cells in a confluent monolayer autonomously. As far as i'm aware this
would be near impossible with brightfield techniques so i have
suggested the cells are fixed and stained but i was wondering which
structures would be best to label to give us the best chance of being
able to do the analysis using ImageJ. I was thinking the nucleus and
some kind of cell junction marker would be best. I'm not sure a
cytoplasmic marker would add much if the cells are all touching.
As far as analysis goes, my knowledge of segmentation and separating
objects goes as far as the basic thresholding and morphological
operators erosion, dilation, watershed etc. Are there any good
specific tools/plugins for this kind of work? And are there other
more advanced methods of segmentation of separating touching objects
available? Perhaps something like level sets would be good for this? I
think the basis of my approach would be nucleus identification to
pinpoint a single cell, cell junction enhancement and final
segmentation of some kind.
Thanks for the advice,
Matt
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