Re: Segmentation of confluent cells

Posted by PEARSON Matthew on May 19, 2014; 3:10pm
URL: http://imagej.273.s1.nabble.com/Segmentation-of-confluent-cells-tp5007785p5007789.html

Hi Johannes,

I'm getting ahead of myself, the cells haven't been prepared for  
imaging yet.  I was asking pre-experiment in case there is anything we  
could do sample prep-wise to aid the analysis once the imaging is  
completed.  What i don't want is for the user to prepare the cells and  
then it turn out that we can't do anything with them, rubbish in,  
rubbish out as they say and its always good to think ahead.  I can  
post some images when we get to that stage.

Thanks,

Matt







On 19 May 2014, at 13:54, Johannes Schindelin wrote:

> Hi Matthew,
>
> On Mon, 19 May 2014, Matthew Pearson wrote:
>
>> I've been asked whether its possible to measure the area of  
>> individual cells
>> in a confluent monolayer autonomously.
>
> You will find that other scientists have a much easier time helping  
> you if
> you provide them with example images.
>
> Ciao,
> Johannes
>

--
Matt Pearson
Microscopy and Imaging Facility
MRC Human Genetics Unit
IGMM
University of Edinburgh
Crewe Road
Edinburgh
EH4 2XU

Tel: [phone]







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