Posted by
Cammer, Michael on
May 19, 2014; 3:27pm
URL: http://imagej.273.s1.nabble.com/Segmentation-of-confluent-cells-tp5007785p5007790.html
Depends 1. on what you want to do with the segmentation and 2.) whether it really has to be automated. In the first case, maybe labeling the nuclei would be enough to mark which cell is which. But if you want to see precise edges of cells, this won't work. So 2.) sometimes labeled cells, such as constitutive GFP or CSFE or cells with the membranes labeled can be separated, or for confluent cells maybe a protein at cell-cell contacts. But in most cases, not. Which brings up how important the experiment is and how lazy the researchers are. Sometimes tracing is the way to go if you want the answer. If tracing 100 cells is too difficult for someone, maybe research is the wrong field to be in...
===========================================================================
Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell: 914-309-3270 Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy &
http://www.med.nyu.edu/skirball-lab/dustinlab/-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Matthew Pearson
Sent: Monday, May 19, 2014 11:11 AM
To:
[hidden email]
Subject: Re: Segmentation of confluent cells
Hi Johannes,
I'm getting ahead of myself, the cells haven't been prepared for imaging yet. I was asking pre-experiment in case there is anything we could do sample prep-wise to aid the analysis once the imaging is completed. What i don't want is for the user to prepare the cells and then it turn out that we can't do anything with them, rubbish in, rubbish out as they say and its always good to think ahead. I can post some images when we get to that stage.
Thanks,
Matt
On 19 May 2014, at 13:54, Johannes Schindelin wrote:
> Hi Matthew,
>
> On Mon, 19 May 2014, Matthew Pearson wrote:
>
>> I've been asked whether its possible to measure the area of
>> individual cells in a confluent monolayer autonomously.
>
> You will find that other scientists have a much easier time helping
> you if you provide them with example images.
>
> Ciao,
> Johannes
>
--
Matt Pearson
Microscopy and Imaging Facility
MRC Human Genetics Unit
IGMM
University of Edinburgh
Crewe Road
Edinburgh
EH4 2XU
Tel: 0131 467 8425
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html