> Depends 1. on what you want to do with the segmentation and 2.)
> whether it really has to be automated. In the first case, maybe
> labeling the nuclei would be enough to mark which cell is which.
> But if you want to see precise edges of cells, this won't work. So
> 2.) sometimes labeled cells, such as constitutive GFP or CSFE or
> cells with the membranes labeled can be separated, or for confluent
> cells maybe a protein at cell-cell contacts. But in most cases,
> not. Which brings up how important the experiment is and how lazy
> the researchers are. Sometimes tracing is the way to go if you want
> the answer. If tracing 100 cells is too difficult for someone,
> maybe research is the wrong field to be in...
> =
> =
> =
> =
> =
> ======================================================================
> Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute,
> NYU Langone Medical Center
> Cell: 914-309-3270 Lab: 212-263-3208
>
http://ocs.med.nyu.edu/microscopy &
http://www.med.nyu.edu/skirball-lab/dustinlab/>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf
> Of Matthew Pearson
> Sent: Monday, May 19, 2014 11:11 AM
> To:
[hidden email]
> Subject: Re: Segmentation of confluent cells
>
> Hi Johannes,
>
> I'm getting ahead of myself, the cells haven't been prepared for
> imaging yet. I was asking pre-experiment in case there is anything
> we could do sample prep-wise to aid the analysis once the imaging is
> completed. What i don't want is for the user to prepare the cells
> and then it turn out that we can't do anything with them, rubbish
> in, rubbish out as they say and its always good to think ahead. I
> can post some images when we get to that stage.
>
> Thanks,
>
> Matt
>
>
>
>
>
>
>
> On 19 May 2014, at 13:54, Johannes Schindelin wrote:
>
>> Hi Matthew,
>>
>> On Mon, 19 May 2014, Matthew Pearson wrote:
>>
>>> I've been asked whether its possible to measure the area of
>>> individual cells in a confluent monolayer autonomously.
>>
>> You will find that other scientists have a much easier time helping
>> you if you provide them with example images.
>>
>> Ciao,
>> Johannes
>>
>
>
>
>
>
>
>
>
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