http://imagej.273.s1.nabble.com/Segmentation-of-confluent-cells-tp5007785p5007805.html
> Hi all,
>
> I've been asked whether its possible to measure the area of individual
> cells in a confluent monolayer autonomously. As far as i'm aware this
> would be near impossible with brightfield techniques so i have suggested
> the cells are fixed and stained but i was wondering which structures would
> be best to label to give us the best chance of being able to do the
> analysis using ImageJ. I was thinking the nucleus and some kind of cell
> junction marker would be best. I'm not sure a cytoplasmic marker would add
> much if the cells are all touching.
>
> As far as analysis goes, my knowledge of segmentation and separating
> objects goes as far as the basic thresholding and morphological operators
> erosion, dilation, watershed etc. Are there any good specific
> tools/plugins for this kind of work? And are there other more advanced
> methods of segmentation of separating touching objects available? Perhaps
> something like level sets would be good for this? I think the basis of my
> approach would be nucleus identification to pinpoint a single cell, cell
> junction enhancement and final segmentation of some kind.
>
> Thanks for the advice,
>
> Matt
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