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Re: Segmentation of confluent cells

Posted by Unruh, Jay-2 on May 21, 2014; 1:54pm
URL: http://imagej.273.s1.nabble.com/Segmentation-of-confluent-cells-tp5007785p5007827.html

Note that for many confluent cell layers, the cells extend beneath one another to a fairly significant extent.  The "boundary" will underestimate the area by a fairly significant amount.  Given this uncertainty and the difficulty in segmenting the boundaries anyway, you are probably better off simply using the distance to the neighboring nuclei as an estimate of cell size.  Voronoi analysis will give you a slightly more sophisticated version of this.

Jay

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Cammer, Michael
Sent: Wednesday, May 21, 2014 8:49 AM
To: [hidden email]
Subject: Re: Segmentation of confluent cells

When I mentioned cell-cell junction proteins, I was thinking of something like e-cadherin.
The red in https://www.flickr.com/photos/mcammer/2234624761/

===========================================================================
Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell:  914-309-3270   Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/

From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Matthew Pearson
Sent: Wednesday, May 21, 2014 7:40 AM
To: [hidden email]
Subject: Re: Segmentation of confluent cells

Hi guys,

Thanks for your responses.  I'll look into region growing algorithms used in ImageJ.  I now a least know that this isn't particularly straight forward.  I'll make sure i'm there for the image capture so the images can be as good as possible and we'll take it from there.

cheers,

Matt

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