Posted by Aryeh Weiss on May 22, 2014; 11:03am URL: http://imagej.273.s1.nabble.com/Re-Crop-cell-and-remove-all-background-tp5007855.html
Seems like you need:
Edit>Clear Outside
which you can apply after you create a closed ROI
--aryeh
On 5/22/14, 1:43 PM, gwenywar wrote:
> Hello,
>
> I have a tiff movie of a few fluorescent cells and I need to crop out one
> cell. I can make a selection of one cell and run the crop command, however
> this gives me a new tiff movie with a rectangular shape.
>
> I can still see the edges of other cells but I need anything outside my
> selected cell to be black. I have tried different things with overlays and
> masks but can't get it to work.
>
> Does anyone have any ideas?
>
> I attached the file with the cell, which I drew around to select my region
> of interest and anything outside that region of interest, should be black.
> Please note that I need to apply this to the whole movie and not just a
> single image.
>
> Thanks for your help!
>
> Cheers
>
> PS: If the uploaded image doesn't work, here is a link:
> https://www.dropbox.com/s/w3p2k2y566ytrwk/Sample%20file.JPG >
>
>
>
> Sample_file.JPG
> <http://imagej.1557.x6.nabble.com/file/n5007852/Sample_file.JPG>
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Crop-cell-and-remove-all-background-tp5007852.html > Sent from the ImageJ mailing list archive at Nabble.com.
>
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--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel