Re: pzfociez macro

Posted by pavanbn on
URL: http://imagej.273.s1.nabble.com/pzfociez-macro-tp5007318p5007862.html


Dear Ion C. Andronache,

The PDF file you sent was a bit confusing for a beginner, I was able to make the mask of my DAPI image, and then combine it with my H2AX staining - after then how do I proceed to count the foci per nuclei?

I clicked on the 'Fluorecence intensity measurement and counting' then on 'FociRB', it then open a dialog box to select the image, I select my H2AX staining picture - and it says 'File is not in a supported format, reader plugin not available, or it was not found'. My image was in .tiff format and was 8bit color image.

Also when I combined the mask of DAPI with H2AX, the outlines appeared around the nuclei (on my H2AX image). Then I chose the 'Find Maxima' option, all the foci inside the nucleus where marked and it counted the foci - but I want it for each nuclei.. Please help...

Just point out to a video if its available, or simply outline the steps I should follow.

Many thanks,
Pavan