Re: multichannel kymographs

Posted by Rasband, Wayne (NIH/NIMH) [E] on
URL: http://imagej.273.s1.nabble.com/multichannel-kymographs-tp5007878p5007885.html

On May 23, 2014, at 11:00 AM, Jeff Spector wrote:

> Greetings,
> I have a stack that is 1800 frames and composed of 2 channels. I would like
> to draw a line and get the kymographs for each channel individually. Is
> there a way to do this without having to split the channels and generate
> the kymograph for each channel by hand?  If I try to make a kymograph of
> the original 1800 frame, 2 channel stack, I end up getting a 3600 line long
> kymograph where the channels are intersperesed, i.e. it goes ch1 ch2 ch1
> ch2 etc..
> I would like them to be separated ( and overlayed on each other if
> possible). Does this tool exist ?

The Image>Stacks>Reslice command in the latest ImageJ daily build (1.49b8) can create
multichannel kymographs from time-lapse 4D hyperstacks (c>1,z=1,t>1). To make a kymograph,  create a line selection, press "/" (shortcut for Reslice) and click "OK" in the dialog (defaults are fine). There is a kymograph tutorial at

   http://fiji.sc/Generate_and_exploit_Kymographs

-wayne

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