Re: multichannel kymographs

Posted by Jeff Spector-2 on
URL: http://imagej.273.s1.nabble.com/multichannel-kymographs-tp5007878p5007897.html

On Fri, May 23, 2014 at 2:31 PM, Rasband, Wayne (NIH/NIMH) [E] <
[hidden email]> wrote:

> On May 23, 2014, at 11:00 AM, Jeff Spector wrote:
>
> > Greetings,
> > I have a stack that is 1800 frames and composed of 2 channels. I would
> like
> > to draw a line and get the kymographs for each channel individually. Is
> > there a way to do this without having to split the channels and generate
> > the kymograph for each channel by hand?  If I try to make a kymograph of
> > the original 1800 frame, 2 channel stack, I end up getting a 3600 line
> long
> > kymograph where the channels are intersperesed, i.e. it goes ch1 ch2 ch1
> > ch2 etc..
> > I would like them to be separated ( and overlayed on each other if
> > possible). Does this tool exist ?
>
> The Image>Stacks>Reslice command in the latest ImageJ daily build (1.49b8)
> can create
> multichannel kymographs from time-lapse 4D hyperstacks (c>1,z=1,t>1). To
> make a kymograph,  create a line selection, press "/" (shortcut for
> Reslice) and click "OK" in the dialog (defaults are fine). There is a
> kymograph tutorial at
>
>    http://fiji.sc/Generate_and_exploit_Kymographs
>
> -wayne
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>



This works great! Thanks!. Is the thickness of the line of the kymograph
defined by the thickness of the line tool used for drawing the roi, or is
it doing only doing a 1 pixel wide line? (is this adjustable?) thanks!

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html