How can we Distinguish In/Out of focus cells in confocal microscope?

Posted by Mohamed Tleis on May 25, 2014; 1:54pm
URL: http://imagej.273.s1.nabble.com/How-can-we-Distinguish-In-Out-of-focus-cells-in-confocal-microscope-tp5007911.html

Dear ImageJ members,

We have two channel images one of bright-field and one of Green
Fluoresence Protein, acquired by a confocal microscope. Recently we are
concerned about some cells having less fluoresent due to the fact that
it is out of focus. In the bright-field channel it is a bit hard to
distinguish between the in and out of focus cells. I wonder whether any
of you came across a similar problem or knows of some literature about it.

Thank you in Advance for your answers.


Best Regards,
M. Tleis
Phd. Candidate
LIACS, Leiden University
The Netherlands.

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